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Mosaic Analysis in the Drosophila melanogaster Ovary

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Book cover Drosophila Oogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1328))

Abstract

Drosophila melanogaster oogenesis is a versatile model system used to address many important questions of cell and developmental biology such as stem cell regulation, cell determination, cell polarization, cell–cell signaling, cell–cell adhesion, and cell-cycle regulation. The ovary is composed of germline and somatic cells of different origins and functions. Mosaic analysis using the powerful genetic tools available in Drosophila melanogaster allows deciphering the contribution of each cell type in the different processes leading to the formation of a mature egg. Germ cells and follicle cells are produced by actively dividing stem cells, which permit the use of recombinases, such as FLP, to generate genetic mosaics using mitotic recombination. This chapter summarizes the different methods used to create genetic mosaics in the germline and in somatic cells of adult ovaries. We briefly introduce the morphology and development of the adult female ovary. We then describe in practical terms how to generate mosaics with examples of cross schemes and recombining strains. We also explain how to identify the appropriate progeny and how to prepare clonal tissues for phenotypic analysis.

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Acknowledgments

T.R. is supported by an FRM Ingenieur Fellowship (n° ING20140129247), and the J.R.H. lab is funded by CNRS, Ville de Paris, and FSER (Schlumberger).

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Authors and Affiliations

  1. Department of Genetics and Developmental Biology, Institut Curie, 26 rue d’Ulm, 75248, Paris, France

    Thomas Rubin & Jean-René Huynh

  2. CNRS UMR3215, 75248, Paris, France

    Thomas Rubin & Jean-René Huynh

  3. Inserm U934, 75248, Paris, France

    Thomas Rubin & Jean-René Huynh

Authors
  1. Thomas Rubin
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  2. Jean-René Huynh
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Correspondence to Jean-René Huynh .

Editor information

Editors and Affiliations

  1. Biological Sciences Department, Hunter College, New York, New York, USA

    Diana P. Bratu

  2. School of Arts and Sciences, Department of Biology,, Jamaica, New York, USA

    Gerard P. McNeil

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Rubin, T., Huynh, JR. (2015). Mosaic Analysis in the Drosophila melanogaster Ovary. In: Bratu, D., McNeil, G. (eds) Drosophila Oogenesis. Methods in Molecular Biology, vol 1328. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2851-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-2851-4_3

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2850-7

  • Online ISBN: 978-1-4939-2851-4

  • eBook Packages: Springer Protocols

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