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Bioinformatics Analysis to Identify RNA–Protein Interactions in Oogenesis

  • Ravinder SinghEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1328)

Abstract

Hundreds of RNA-binding proteins are known, but the biological functions are known for only a few of them. They regulate various aspects of RNA processing or biogenesis such as splicing, polyadenylation, and translation. Here I describe a bioinformatics approach that we developed to identify potential new mRNA target(s) of the Drosophila master sex-switch protein Sex-lethal (SXL) by combining computational analysis with genetic and biochemical investigation. This approach could be used to identify new RNA–protein interactions during oogenesis in the female germline and should be applicable to numerous other posttranscriptional regulatory events.

Key words

Sex determination RNA-binding proteins Germline Oogenesis Polyadenylation Translation 

Notes

Acknowledgements

I thank Andrew Rahn and Mark Robida for their notes consulted for materials and methods and the American Cancer Society and the Butcher Foundation for grants to RS that supported this work.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Molecular, Cellular and Developmental BiologyUniversity of Colorado at BoulderBoulderUSA

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