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Analysis of Cell Cycle Switches in Drosophila Oogenesis

  • Dongyu Jia
  • Yi-Chun Huang
  • Wu-Min DengEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1328)

Abstract

The study of Drosophila oogenesis provides invaluable information about signaling pathway regulation and cell cycle programming. During Drosophila oogenesis, a string of egg chambers in each ovariole progressively develops toward maturity. Egg chamber development consists of 14 stages. From stage 1 to stage 6 (mitotic cycle), main-body follicle cells undergo mitotic divisions. From stage 7 to stage 10a (endocycle), follicle cells cease mitosis but continue three rounds of endoreduplication. From stage 10b to stage 13 (gene amplification), instead of whole genome duplication, follicle cells selectively amplify specific genomic regions, mostly for chorion production. So far, Drosophila oogenesis is one of the most well studied model systems used to understand cell cycle switches, which furthers our knowledge about cell cycle control machinery and sheds new light on potential cancer treatments. Here, we give a brief summary of cell cycle switches, the associated signaling pathways and factors, and the detailed experimental procedures used to study the cell cycle switches.

Keywords

Endocycle Mitotic cycle Gene amplification Drosophila oogenesis Follicle cell 

Notes

Acknowledgements

We would like to thank Gabriel Calvin, Gengqiang Xie, Allison Jevitt, and Sarayu Row for critical reading of and comments on the manuscript. Special thanks to Jianjun Sun and other former and current members of the Deng lab for developing protocols on this topic; W.-M.D. is supported by NIH grant R01GM072562.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Biological ScienceFlorida State UniversityTallahasseeUSA

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