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Visualizing Cytoophidia Expression in Drosophila Follicle Cells via Immunohistochemistry

  • Ömür Y. Tastan
  • Ji-Long LiuEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1328)

Abstract

We describe a user-friendly immunohistochemical approach for the detection of protein localization in Drosophila ovaries, here focusing on CTP synthase. This approach mainly uses fluorescently labeled antibodies to detect single, double, or multiple antigens. We provide a step-by-step protocol with detailed notes and tips, a simplified method that can also be adapted to detect protein localization beyond Drosophila ovaries.

Keywords

Drosophila Ovary Antibody Antigen Immunohistochemistry Follicle cell Cytoophidium 

Notes

Acknowledgments

The authors wish to thank the Drosophila community for generously sharing reagents and antibodies. The research in the Liu Laboratory was supported by the UK Medical Research Council.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.MRC Functional Genomics Unit, Department of Physiology, Anatomy and GeneticsUniversity of OxfordOxfordUK

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