Abstract
Avian species are among the most diverse vertebrates on our planet and significantly contribute to the balance of the ecology. They are also important food source and serve as a central animal model to decipher developmental biology and disease principles. Derivation of induced pluripotent stem cells (iPSCs) from avian species would enable conservation of genetic diversity as well as offer a valuable cell source that facilitates the use of avian models in many areas of basic and applied research. In this chapter, we describe methods used to successfully reprogram quail fibroblasts into iPSCs by using human transcription factors and the techniques critical to the characterization of their pluripotency.
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Acknowledgements
This work was supported by the Bill and Melinda Gates Foundation and the Guangxi International Collaboration Project (1347004-6).
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Lu, Y., West, F.D., Jordan, B.J., Beckstead, R.B., Jordan, E.T., Stice, S.L. (2015). Generation of Avian Induced Pluripotent Stem Cells. In: Verma, P., Sumer, H. (eds) Cell Reprogramming. Methods in Molecular Biology, vol 1330. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2848-4_9
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DOI: https://doi.org/10.1007/978-1-4939-2848-4_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2847-7
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