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Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer

  • Protocol
Cell Reprogramming

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1330))

Abstract

In 2003, the first wild felid was produced by interspecies somatic cell nuclear transfer. Since then other wild felid clone offspring have been produced by using the same technique with minor modifications. This chapter describes detailed protocols used in our laboratory for (1) the isolation, culture, and preparation of fibroblast cells as donor nucleus, and (2) embryo reconstruction with domestic cat enucleated oocytes to produce cloned embryos that develop to the blastocyst stage in vitro and, after transfer into synchronized recipients, establish successful pregnancies.

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Author information

Authors and Affiliations

  1. Audubon Nature Center for Research of Endangered Species, 14001 River Road, New Orleans, LA, 70131, USA

    Martha C. Gómez & C. Earle Pope

Authors
  1. Martha C. Gómez
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  2. C. Earle Pope
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Corresponding author

Correspondence to Martha C. Gómez .

Editor information

Editors and Affiliations

  1. Stem Cell and Genetic Engineering Group, Department of Materials Engineering Faculty of Engineering, Monash University, Clayton, Victoria, Australia

    Paul J. Verma

  2. Swinburne University of Technology, Hawthorn, Victoria, Australia

    Huseyin Sumer

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Gómez, M.C., Pope, C.E. (2015). Cloning Endangered Felids by Interspecies Somatic Cell Nuclear Transfer. In: Verma, P., Sumer, H. (eds) Cell Reprogramming. Methods in Molecular Biology, vol 1330. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2848-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2848-4_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2847-7

  • Online ISBN: 978-1-4939-2848-4

  • eBook Packages: Springer Protocols

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