Abstract
Transgenesis in model organisms is necessary to determine the function, expression, and subcellular localization of gene products. In Caenorhabditis elegans, injected DNA can be propagated as multicopy extrachromosomal arrays but transgenes in arrays are mosaic, over-expressed in some tissues and silenced in the germline. Here, a method to insert a transgene into a specific genomic location called Mos1-mediated single-copy insertion (MosSCI) is described. Single-copy insertion allows transgene expression at levels that approximate endogenous gene expression as well as expression in the germline.
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Acknowledgements
Strains with Mos1 elements were generated by the nemaGENETAG consortium. This work was supported by a postdoctoral fellowships from the Lundbeck and Carlsberg Foundations. The research was performed in the laboratory of Erik M. Jorgensen at University of Utah and supported by NIH grant 1R01GM095817 (E.M.J.) and Howard Hughes Medical Institute (E.M.J.).
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Frøkjær-Jensen, C. (2015). Transposon-Assisted Genetic Engineering with Mos1-Mediated Single-Copy Insertion (MosSCI). In: Biron, D., Haspel, G. (eds) C. elegans. Methods in Molecular Biology, vol 1327. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4939-2842-2_5
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DOI: https://doi.org/10.1007/978-1-4939-2842-2_5
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4939-2841-5
Online ISBN: 978-1-4939-2842-2
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