Abstract
The quantification of single cell interferon-gamma (IFN-γ) release for assessing cellular immune responses using the Enzyme-linked immunospot (ELISPOT) assay is an invaluable technique in immunology. Peripheral blood mononuclear cells (PBMC) are stimulated in vitro with recombinant proteins, peptides and recently whole malaria organisms. Stimulation may be short term (20–36 h) or long term (cultured ELISpot, up to 7 days). ELISpot is also able to quantify other cytokines secreted by antigen-specific T-cells, such as interleukin-2, interleukin-5, and other interleukins. ELISpot is playing an important role especially in vaccine research studies.
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Acknowledgments
The following have made major contributions to the development and use of this assay: Harini Ganeshan, Maria Belmonte, Jun Huang, Esteban Abot, and Arnel Belmonte, and helped write this chapter with Michael R. Hollingdale.
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Sedegah, M. (2015). The Ex Vivo IFN-γ Enzyme-Linked Immunospot (ELISpot) Assay. In: Vaughan, A. (eds) Malaria Vaccines. Methods in Molecular Biology, vol 1325. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2815-6_16
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DOI: https://doi.org/10.1007/978-1-4939-2815-6_16
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2814-9
Online ISBN: 978-1-4939-2815-6
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