Abstract
The agglutination assay is used to determine the ability of antibodies to recognize parasite variant antigens on the surface of Plasmodium falciparum-infected erythrocytes. In this technique, infected erythrocytes are selectively labelled with a DNA-binding fluorescent dye and mixed with antibodies of interest to allow antibody–surface antigen binding. Recognition of surface antigens by the antibodies can result in the formation of agglutinates containing multiple parasite-infected erythrocytes. These can be viewed and quantified using a fluorescence microscope.
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Acknowledgements
This work was supported by a Wellcome Trust Grant for a 4-Year Ph.D. in Infection, Immunology, and Translational Medicine (for J.T.).
This book chapter was published with permission from the director of KEMRI.
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Tan, J., Bull, P.C. (2015). Agglutination Assays of the Plasmodium falciparum-Infected Erythrocyte. In: Vaughan, A. (eds) Malaria Vaccines. Methods in Molecular Biology, vol 1325. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2815-6_10
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DOI: https://doi.org/10.1007/978-1-4939-2815-6_10
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