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Computing Conformational Free Energies of iGluR Ligand-Binding Domains

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Ionotropic Glutamate Receptor Technologies

Part of the book series: Neuromethods ((NM,volume 106))

Abstract

Ionotropic glutamate receptors (iGluRs) transduce chemical signals at synapses into electrical impulses. This function relies on concerted conformational changes that are propagated among the linked domains of the tetrameric protein assembly making up each receptor. A key conformational change is the closure of the ligand-binding domain (LBD) upon agonist binding, which eventually gates the transmembrane ion channel domain. The free energy that becomes available for gating transitions is governed by the LBD free energy landscapes for apo and ligand-bound states. These landscapes describe the thermodynamic equilibrium among various LBD conformations. Delineating these landscapes is essential for understanding the molecular driving forces underlying iGluR function. Molecular dynamics free energy simulations offer a means for estimating these quantities, which are difficult to extract from experimental results alone. Here, we describe the process of carrying out a free energy computation using an umbrella sampling strategy for characterizing large-scale conformational changes in iGluR LBDs.

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Authors and Affiliations

  1. Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Alvin Yu, Tyler Wied, John Belcher & Albert Y. Lau

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  1. Alvin Yu
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  2. Tyler Wied
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  3. John Belcher
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  4. Albert Y. Lau
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Corresponding author

Correspondence to Albert Y. Lau .

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Editors and Affiliations

  1. Department of Biochemistry, University at Buffalo, Buffalo, New York, USA

    Gabriela K. Popescu

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Yu, A., Wied, T., Belcher, J., Lau, A.Y. (2016). Computing Conformational Free Energies of iGluR Ligand-Binding Domains. In: Popescu, G. (eds) Ionotropic Glutamate Receptor Technologies. Neuromethods, vol 106. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2812-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-2812-5_9

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2811-8

  • Online ISBN: 978-1-4939-2812-5

  • eBook Packages: Springer Protocols

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