Abstract
During their development, human T cells undergo similar genomic changes and pass through the same developmental checkpoints as developing thymocytes in the mouse. The difference between both species, however, is that some of these developmental stages are characterized by different phenotypic markers and as a result, evidence emerges that the molecular regulation of human T cell development subtly differs from the mouse [1–4]. In this chapter, we describe in detail how the different stages of human T cell development can be characterized and isolated using specific surface markers.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Taghon T, Waegemans E, Van de Walle I (2012) Notch signaling during human T cell development. Curr Top Micobiol Immunol 360:75–97
Haddad R, Guimiot F, Sic E et al (2006) Dynamics of thymus-colonizing cells during human development. Immunity 24:217–230
Hao QL, George AA, Zhu J et al (2008) Human intrathymic lineage commitment is marked by differential CD7 expression: identification of CD7- lympho-myeloid thymic progenitors. Blood 111:1318–1326
Taghon T, Rothenberg EV (2008) Molecular mechanisms that control mouse and human TCR-αβ and TCR-γδ T cell development. Semin Immunopathol 30:383–398
Weerkamp F, Baert MR, Brugman MH et al (2006) Human thymus contains multipotent progenitors with T/B lymphoid, myeloid, and erythroid lineage potential. Blood 107:3131–3137
Ratajczak MZ (2008) Phenotypic and functional characterization of hematopoietic stem cells. Curr Opin Hematol 15:293–300
Dik WA, Pike-Overzet K, Weerkamp F et al (2005) New insights on human T cell development by quantitative T cell receptor gene rearrangement studies and gene expression profiling. J Exp Med 201:1715–1723
Carrasco YR, Trigueros C, Ramiro AR et al (1999) Beta-selection is associated with the onset of CD8beta chain expression on CD4(+)CD8alphaalpha(+) pre-T cells during human intrathymic development. Blood 94:3491–3498
Joachims ML, Chain JL, Hooker SW et al (2006) Human alpha beta and gamma delta thymocyte development: TCR gene rearrangements, intracellular TCR beta expression, and gamma delta developmental potential–differences between men and mice. J Immunol 176:1543–1552
Taghon T, Van de Walle I, De Smet G et al (2009) Notch signaling is required for proliferation but not for differentiation at a well-defined beta-selection checkpoint during human T-cell development. Blood 113:3254–3263
Plum J, De Smedt M, Leclercq G et al (2008) Human intrathymic development: a selective approach. Semin Immunopathol 30:411–423
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Van de Walle, I., Davids, K., Taghon, T. (2016). Characterization and Isolation of Human T Cell Progenitors. In: Bosselut, R., S. Vacchio, M. (eds) T-Cell Development. Methods in Molecular Biology, vol 1323. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2809-5_19
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2809-5_19
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2808-8
Online ISBN: 978-1-4939-2809-5
eBook Packages: Springer Protocols