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Cell-Penetrating Peptides pp 261–277Cite as

Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery In Vitro

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1324))

Abstract

There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.

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Acknowledgements

María García-Díaz is acknowledged for supplying the protocols for the mucus preparation and the permeation study using Caco-2 cell monolayers supplemented with biosimilar mucus. The research leading to these results has received support from the Innovative Medicines Initiative Joint Undertaking under grant agreement no. 115363 resources which are composed of financial contribution from the European Union’s Seventh Framework Programme (FP7/2007–2013) and EFPIA companies’ in kind contribution. Additionally, the work was financially supported by the Drug Research Academy (University of Copenhagen), The Danish Agency for Science, Technology & Innovation, and the Novo Nordisk Foundation Center for Protein Research.

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Authors and Affiliations

  1. Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Stine Rønholt & Mie Kristensen

  2. Section for Biologics, Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark

    Hanne Mørck Nielsen

Authors
  1. Stine Rønholt
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  2. Mie Kristensen
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  3. Hanne Mørck Nielsen
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Corresponding author

Correspondence to Hanne Mørck Nielsen .

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Editors and Affiliations

  1. Department of Neurochemistry, Stockholm University, Stockholm, Sweden

    Ülo Langel

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Rønholt, S., Kristensen, M., Nielsen, H.M. (2015). Cell-Penetrating Peptides as Carriers for Transepithelial Drug Delivery In Vitro. In: Langel, Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology, vol 1324. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2806-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-2806-4_17

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2805-7

  • Online ISBN: 978-1-4939-2806-4

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