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SCARA Involvement in the Uptake of Nanoparticles Formed by Cell-Penetrating Peptides

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Cell-Penetrating Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1324))

Abstract

The investigation of uptake mechanisms for cell-penetrating peptides (CPPs) is and has been an ongoing project for as long as the peptides have been known, a time period that now spans over two decades. The ultimate answer is yet to be revealed and the current understanding is that no “one” mechanism will ever be found. The reason for this is that the uptake mechanism seems to be dependent on a multitude of factors that include which CPP, what cells are used, whether or not there is cargo and what the cargo is. CPPs are capable of delivering a variety of bio-macromolecules that are by themselves unable to enter into cells. Our group has reported on many different peptides in recent years, many aimed at delivering various oligonucleotide-based cargoes. These peptides have utilized the inherent positive charge of the peptides and some rationally designed modifications to non-covalently complex oligonucleotides and bring them into cells. In this chapter, we present a brief overview of the current proposals for the uptake mechanisms of CPPs and describe methods for detecting and evaluating the role of scavenger receptor class A receptors in the uptake of non-covalent cell-penetrating peptide:oligonucleotide complexes.

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Acknowledgements

This work was supported by The Swedish Research Council.

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Authors and Affiliations

  1. Department of Neurochemistry, Stockholm University, Stockholm, 10691, Sweden

    Henrik Helmfors, Staffan Lindberg & Ülo Langel

  2. Laboratory of Molecular Biotechnology, Institute of Technology, Tartu University, Tartu, Estonia

    Ülo Langel

Authors
  1. Henrik Helmfors
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  2. Staffan Lindberg
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  3. Ülo Langel
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Corresponding author

Correspondence to Henrik Helmfors .

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Editors and Affiliations

  1. Department of Neurochemistry, Stockholm University, Stockholm, Sweden

    Ülo Langel

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Helmfors, H., Lindberg, S., Langel, Ü. (2015). SCARA Involvement in the Uptake of Nanoparticles Formed by Cell-Penetrating Peptides. In: Langel, Ü. (eds) Cell-Penetrating Peptides. Methods in Molecular Biology, vol 1324. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2806-4_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2806-4_11

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2805-7

  • Online ISBN: 978-1-4939-2806-4

  • eBook Packages: Springer Protocols

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