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Hedgehog Signaling Protocols pp 187–198Cite as

Determination and Analysis of Cellular Metabolic Changes by Noncanonical Hedgehog Signaling

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1322))

Abstract

Hedgehog is a morphogen essential for body patterning and proper embryonic development from flies to humans. Thought quiescent in adults, its inappropriate reactivation is associated with many disparate genetic and sporadic types of human cancers. Recent findings have demonstrated a key, yet unexpected, role of the Hedgehog signaling pathway in metabolic control. Here, we describe a panel of methods to determine and analyze cellular and organismal metabolic changes downstream of the Hedgehog signaling pathway.

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Acknowledgements

JAP acknowledges generous support from the DFG, ERC, EU-FP7, BMBF (DEEP), and the MPG.

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Authors and Affiliations

  1. Institute of Experimental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Ingolstädter Landstrasse 1, 85764, Neuherberg, Munich, Germany

    Raffaele Teperino

  2. Max Planck Institute of Immunobiology and Epigenetics, Stuebeweg 51, 79108, Freiburg im Breisgau, Germany

    John Andrew Pospisilik

Authors
  1. Raffaele Teperino
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  2. John Andrew Pospisilik
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Corresponding author

Correspondence to John Andrew Pospisilik .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    Natalia A. Riobo

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Teperino, R., Pospisilik, J.A. (2015). Determination and Analysis of Cellular Metabolic Changes by Noncanonical Hedgehog Signaling. In: Riobo, N. (eds) Hedgehog Signaling Protocols. Methods in Molecular Biology, vol 1322. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2772-2_16

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  • DOI: https://doi.org/10.1007/978-1-4939-2772-2_16

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2771-5

  • Online ISBN: 978-1-4939-2772-2

  • eBook Packages: Springer Protocols

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