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Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate

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Hedgehog Signaling Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1322))

Abstract

Here we describe the preparation of a delivery vehicle for controlled release of Sonic hedgehog (Shh). The vehicle contains a synthetic polycation and heparin which interact by polyvalent charge attraction and rapidly self-assemble into liquid coacervate droplets. The coacervate loads Shh with high efficiency, protects its bioactivity, and provides sustained and localized release at the site of application. Shh coacervate may be injected directly or coated onto a polymeric scaffold for tissue engineering approaches, as described here.

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Author information

Authors and Affiliations

  1. Department of Bioengineering, University of Pittsburgh, 302 Benedum Hall, 3700 O’Hara St.,, Pittsburgh, PA, 15219, USA

    Noah Ray Johnson & Yadong Wang Ph.D.

  2. McGowan Institute for Regenerative Medicine, Pittsburgh, PA, 15219, USA

    Noah Ray Johnson & Yadong Wang Ph.D.

  3. Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, 15219, USA

    Yadong Wang Ph.D.

  4. Department of Surgery, University of Pittsburgh, Pittsburgh, PA, 15219, USA

    Yadong Wang Ph.D.

  5. Department of Mechanical Engineering and Materials Science, University of Pittsburgh, Pittsburgh, PA, 15219, USA

    Yadong Wang Ph.D.

Authors
  1. Noah Ray Johnson
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  2. Yadong Wang Ph.D.
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Corresponding author

Correspondence to Yadong Wang Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Sidney Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, Pennsylvania, USA

    Natalia A. Riobo

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Johnson, N.R., Wang, Y. (2015). Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate. In: Riobo, N. (eds) Hedgehog Signaling Protocols. Methods in Molecular Biology, vol 1322. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2772-2_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2772-2_1

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2771-5

  • Online ISBN: 978-1-4939-2772-2

  • eBook Packages: Springer Protocols

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