Abstract
In vitro reconstitution systems of ribosomal subunits from free ribosomal RNA and ribosomal proteins are helpful tool for studies on the structure, function and assembly of ribosome. Using this system mutant or modified ribosomal proteins or ribosomal RNA can be incorporated into ribosomal subunits for studying ribosome structure and function. Developing the protocol for reconstitution of 30S subunits from an extreme thermophilic bacterium Thermus thermophilus can be beneficial especially for structural studies, as proteins and nucleic acids from this organism are very stable and crystallize easier than those from mesophilic organisms.
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Acknowledgements
We are grateful to A.S. Spirin for his support of early stages on the study of Thermus thermophilus ribosomes; V.A. Shirokov for stimulating discussions. This work was supported by Russian Academy of Science.
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Agalarov, S., Yusupov, M., Yusupova, G. (2016). Reconstitution of Functionally Active Thermus thermophilus 30S Ribosomal Subunit from Ribosomal 16S RNA and Ribosomal Proteins. In: Ennifar, E. (eds) Nucleic Acid Crystallography. Methods in Molecular Biology, vol 1320. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2763-0_19
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DOI: https://doi.org/10.1007/978-1-4939-2763-0_19
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