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Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in E. coli

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Glyco-Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1321))

Abstract

The biotechnological relevance of protein glycosylation has exponentially grown in recent years. With the advances in protein glycosylation research, new possibilities for glyco-engineering have arisen, and a wide array of glycans can be designed and potentially transferred to target proteins in the biotechnologically relevant host Escherichia coli. Here we provide insight on how to select the best strains and plasmids. We also describe methods for determination of glycan expression and assembly, protein glycosylation using western blot, and preparation of samples for mass spectrometry.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada

    Nicolas F. Vozza & Mario F. Feldman

Authors
  1. Nicolas F. Vozza
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  2. Mario F. Feldman
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Editor information

Editors and Affiliations

  1. University of Natural Resources and Life Sciences, Vienna, Austria

    Alexandra Castilho

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Vozza, N.F., Feldman, M.F. (2015). Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in E. coli . In: Castilho, A. (eds) Glyco-Engineering. Methods in Molecular Biology, vol 1321. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2760-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-2760-9_5

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2759-3

  • Online ISBN: 978-1-4939-2760-9

  • eBook Packages: Springer Protocols

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