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N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements

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Book cover Glyco-Engineering

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1321))

Abstract

Plants are being developed as a cost-effective production system for biopharmaceuticals in large quantities. Although plants properly fold and assemble complex proteins from human origin, one issue that needs to be addressed is their glycan structure. In the past years we have been witnessing outstanding results in targeted manipulation of the plant N-glycosylation pathway allowing recombinant proteins to be produced with human-type oligosaccharides at large homogeneity. This opens new possibility in manufacturing next-generation biopharmaceuticals.

This review presents a variety of technologies and strategies that are being employed to engineer the plant N-glycosylation, thus pointing to the enormous potential of plants being used as a novel production system with unique features and possibilities.

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Abbreviations

C1GalT1:

Drosophila melanogaster Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1

CMAS:

Human CMP-N-acetylneuraminic acid synthase

CST:

Mouse CMP-sialic acid transporter

CTS:

Cytoplasmic tail, transmembrane domain, and stem

FUT11:

A. thaliana α1,3-fucosyltransferase

FUT8:

Human α1,6-fucosyltransferase

FUT9a:

Human α1,3-fucosyltransferase IXa

GalNAc-T2:

Human polypeptide N-acetylgalactosaminyltransferase

GalT:

Human β1,4-galactosyltransferase

GE:

Pseudomonas aeruginosa or Yersinia enterocolitica GMI, Golgi mannosidase I

GMII:

Golgi mannosidase II

GNE:

Mouse UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine-kinase

GnT-I:

α1,3-Mannosyl-β1,2-N-acetylglucosaminyltransferase I

GnT-II:

α1,6-Mannosyl-β1,2-N-acetylglucosaminyltransferase II

GnT-III:

Human β1,4-mannosyl-β1,4-N-acetylglucosaminyltransferase III

GnT-IV:

Human α1,3-mannosyl-β1,4-N-acetylglucosaminyltransferase IVa

GnT-V:

Human α1,6-mannosyl-β1,6-N-acetylglucosaminyltransferase V

HEXO1 and HEXO3:

Arabidopsis β-N-acetylhexosaminidases 1 and 3

NANS:

Human N-acetylneuraminic acid phosphate-synthase

P4H:

Prolyl-4-hydroxylase

ST:

Rat or human α2,6-sialyltransferase

ST3GalI:

Human α2,3-sialyltransferase

ST6GalNAc4:

Mouse α2,6-sialyltransferase

UDP-GlcNAc/UDP-GalNAc transporter:

UPD-GlcNAc 4-epimerase

UGT:

Caenorhabditis elegans

XylT:

β1,2-Xylosyltransferase

α1,4-FucT:

α1,4-Fucosyltransferase

β1,3-GalT:

β1,3-Galactosyltransferase

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Authors and Affiliations

  1. Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Sciences, Vienna, Austria

    Herta Steinkellner & Alexandra Castilho

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  1. Herta Steinkellner
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  2. Alexandra Castilho
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  1. University of Natural Resources and Life Sciences, Vienna, Austria

    Alexandra Castilho

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Steinkellner, H., Castilho, A. (2015). N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements. In: Castilho, A. (eds) Glyco-Engineering. Methods in Molecular Biology, vol 1321. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2760-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-2760-9_14

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2759-3

  • Online ISBN: 978-1-4939-2760-9

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