Abstract
Site-specific injection of epigenetic modulating drugs into the brain, using neurostereotaxic surgery, is a very valuable experimental tool. This technique enables the pharmacological manipulation of targeted molecules, such as DNMT and HDAC, in specific brain areas, in conscious animals. This chapter presents a step-by-step description of cannula implantation in targeted brain structures and microinjection using neurostereotaxic surgery.
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References
Alt KW, Jeunesse C, Buitrago-Tellez CH, Wachter R, Boes E, Pichler SL (1997) Evidence for stone age cranial surgery. Nature 387(6631):360
Rawlings CE 3rd, Rossitch E Jr (1994) Franz Josef Gall and his contribution to neuroanatomy with emphasis on the brain stem. Surg Neurol 42(3):272–275
Simpson D (2005) Phrenology and the neurosciences: contributions of F. J. Gall and J. G. Spurzheim. ANZ J Surg 75(6):475–482
Webster WG (1973) Assumptions, conceptualizations, and the search for the functions of the brain. Physiol Psychol 1(4):346
Robison RA, Taghva A, Liu CY, Apuzzo ML (2013) Surgery of the mind, mood, and conscious state: an idea in evolution. World Neurosurg 80(3-4):S2–S26
Chen DC, Lin SZ, Fan JR, Lin CH, Lee W, Lin CC et al (2014) Intracerebral implantation of autologous peripheral blood stem cells in stroke patients: a randomized phase II study. Cell Transplant 23:1599
Leiphart JW, Young RM, Shields DC (2014) A historical perspective: stereotactic lesions for the treatment of epilepsy. Seizure 23(1):1–5
Tsuboi T, Watanabe H, Tanaka Y, Ohdake R, Yoneyama N, Hara K, et al (2014) Distinct phenotypes of speech and voice disorders in Parkinson’s disease after subthalamic nucleus deep brain stimulation. J Neurol Neurosurg Psychiatry 86(8):856–864
Fornari RV, Wichmann R, Atsak P, Atucha E, Barsegyan A, Beldjoud H et al (2012) Rodent stereotaxic surgery and animal welfare outcome improvements for behavioral neuroscience. J Vis Exp 59:e3528
Paxinos G, Watson C (2007) The rat brain in stereotaxic coordinates, 6th edn. Academic, London
Paxinos G, Watson C, Pennisi M, Topple A (1985) Bregma, lambda and the interaural midpoint in stereotaxic surgery with rats of different sex, strain and weight. J Neurosci Methods 13(2):139–143
Greenberg BD, Rauch SL, Haber SN (2010) Invasive circuitry-based neurotherapeutics: stereotactic ablation and deep brain stimulation for OCD. Neuropsychopharmacology 35(1):317–336
Netto SM, Silveira R, Coimbra NC, Joca SR, Guimaraes FS (2002) Anxiogenic effect of median raphe nucleus lesion in stressed rats. Prog Neuropsychopharmacol Biol Psychiatry 26(6):1135–1141
Casarotto PC, de Bortoli VC, Correa FM, Resstel LB, Zangrossi H Jr (2010) Panicolytic-like effect of BDNF in the rat dorsal periaqueductal grey matter: the role of 5-HT and GABA. Int J Neuropsychopharmacol 13(5):573–582
LaPlant Q, Vialou V, Covington HE 3rd, Dumitriu D, Feng J, Warren BL et al (2010) Dnmt3a regulates emotional behavior and spine plasticity in the nucleus accumbens. Nat Neurosci 13(9):1137–1143
Sales AJ, Biojone C, Terceti MS, Guimarães FS, Gomes MV, Joca SR (2011) Antidepressant-like effect induced by systemic and intra-hippocampal administration of DNA methylation inhibitors. Br J Pharmacol 164:1711
Tsankova NM, Berton O, Renthal W, Kumar A, Neve RL, Nestler EJ (2006) Sustained hippocampal chromatin regulation in a mouse model of depression and antidepressant action. Nat Neurosci 9(4):519–525
Acknowledgements
The authors acknowledge José Carlos de Aguiar for his outstanding dedication in establishing and improving stereotaxic surgery in our university.
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Sales, A., Biojone, C., Joca, S. (2016). Site-Specific Delivery of Epigenetic Modulating Drugs into the Rat Brain. In: Karpova, N. (eds) Epigenetic Methods in Neuroscience Research. Neuromethods, vol 105. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2754-8_10
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DOI: https://doi.org/10.1007/978-1-4939-2754-8_10
Publisher Name: Humana Press, New York, NY
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