Epitope-Specific Binder Design by Yeast Surface Display

  • Jasdeep K. Mann
  • Sheldon ParkEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1319)


Yeast surface display is commonly used to engineer affinity and design novel molecular interaction. By alternating positive and negative selections, yeast display can be used to engineer binders that specifically interact with the target protein at a defined site. Epitope-specific binders can be useful as inhibitors if they bind the target molecule at functionally important sites. Therefore, an efficient method of engineering epitope specificity should help with the engineering of inhibitors. We describe the use of yeast surface display to design single domain monobodies that bind and inhibit the activity of the kinase Erk-2 by targeting a conserved surface patch involved in protein–protein interaction. The designed binders can be used to disrupt signaling in the cell and investigate Erk-2 function in vivo. The described protocol is general and can be used to design epitope-specific binders of an arbitrary protein.

Key words

Yeast surface display Epitope-specific interaction Monobody Negative design Assay development 



This work was supported by the NSF grant (1053608) to S.P.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Department of Chemical and Biological EngineeringUniversity at BuffaloBuffaloUSA
  2. 2.Bluebird BioSeattleUSA

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