Electrochemical Glucose Biosensor Based on Glucose Oxidase Displayed on Yeast Surface
The conventional enzyme-based biosensor requires chemical or physical immobilization of purified enzymes on electrode surface, which often results in loss of enzyme activity and/or fractions immobilized over time. It is also costly. A major advantage of yeast surface display is that it enables the direct utilization of whole cell catalysts with eukaryote-produced proteins being displayed on the cell surface, providing an economic alternative to traditional production of purified enzymes. Herein, we describe the details of the display of glucose oxidase (GOx) on yeast cell surface and its application in the development of electrochemical glucose sensor. In order to achieve a direct electrochemistry of GOx, the entire cell catalyst (yeast-GOx) was immobilized together with multiwalled carbon nanotubes on the electrode, which allowed sensitive and selective glucose detection.
Key wordsYeast surface display Glucose oxidase Electrochemical biosensor Glucose
This work was supported in part by National Natural Science Foundation of China (91227116, 31200982, 31200598, 21275152, and 21475144), and the Hundred-Talent-Project (No. KSCX2-YW-BR-7), the Chinese Academy of Sciences.
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