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Electrochemical Glucose Biosensor Based on Glucose Oxidase Displayed on Yeast Surface

  • Hongwei Wang
  • Qiaolin Lang
  • Bo Liang
  • Aihua Liu
Part of the Methods in Molecular Biology book series (MIMB, volume 1319)

Abstract

The conventional enzyme-based biosensor requires chemical or physical immobilization of purified enzymes on electrode surface, which often results in loss of enzyme activity and/or fractions immobilized over time. It is also costly. A major advantage of yeast surface display is that it enables the direct utilization of whole cell catalysts with eukaryote-produced proteins being displayed on the cell surface, providing an economic alternative to traditional production of purified enzymes. Herein, we describe the details of the display of glucose oxidase (GOx) on yeast cell surface and its application in the development of electrochemical glucose sensor. In order to achieve a direct electrochemistry of GOx, the entire cell catalyst (yeast-GOx) was immobilized together with multiwalled carbon nanotubes on the electrode, which allowed sensitive and selective glucose detection.

Key words

Yeast surface display Glucose oxidase Electrochemical biosensor Glucose 

Notes

Acknowledgements

This work was supported in part by National Natural Science Foundation of China (91227116, 31200982, 31200598, 21275152, and 21475144), and the Hundred-Talent-Project (No. KSCX2-YW-BR-7), the Chinese Academy of Sciences.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Hongwei Wang
    • 1
    • 2
  • Qiaolin Lang
    • 1
  • Bo Liang
    • 1
  • Aihua Liu
    • 1
  1. 1.Laboratory for Biosensing, Qingdao Institute of Bioenergy & Bioprocess Technology (QIBEBT) and Key Laboratory of Biofuels (QIBEBT)Chinese Academy of SciencesQingdaoChina
  2. 2.State Key Laboratory of Crop Biology, College of AgronomyShandong Agricultural UniversityTai’an, ShandongChina

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