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Enzyme Evolution by Yeast Cell Surface Engineering

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Yeast Surface Display

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1319))

Abstract

Artificial evolution of proteins with the aim of acquiring novel or improved functionality is important for practical applications of the proteins. We have developed yeast cell surface engineering methods (or arming technology) for evolving enzymes. Here, we have described yeast cell surface engineering coupled with in vivo homologous recombination and library screening as a method for the artificial evolution of enzymes such as firefly luciferases. Using this method, novel luciferases with improved substrate specificity and substrate reactivity were engineered.

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Author information

Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, 606-8502, Japan

    Natsuko Miura, Kouichi Kuroda & Mitsuyoshi Ueda

  2. Radiation Oncology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, 20892, USA

    Natsuko Miura

Authors
  1. Natsuko Miura
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  2. Kouichi Kuroda
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  3. Mitsuyoshi Ueda
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Corresponding author

Correspondence to Mitsuyoshi Ueda .

Editor information

Editors and Affiliations

  1. UCSF, San Francisco, California, USA

    Bin Liu

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Miura, N., Kuroda, K., Ueda, M. (2015). Enzyme Evolution by Yeast Cell Surface Engineering. In: Liu, B. (eds) Yeast Surface Display. Methods in Molecular Biology, vol 1319. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2748-7_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2748-7_12

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2747-0

  • Online ISBN: 978-1-4939-2748-7

  • eBook Packages: Springer Protocols

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