Abstract
The generation of hybridoma cell lines by the fusion of splenocytes from immunized mice with immortal myeloma cells is a well-established method for the production of monoclonal antibodies. Although other methods have emerged as an effective alternative for the generation of monoclonal antibodies, the use of hybridoma technology remains a viable technique that is accessible to a wide number of laboratories that perform basic cell biological research. Hybridoma technology represents a relatively simple procedure at minimal cost for the continuous production of native whole immunoglobulins. This chapter will describe the materials and methodologies needed for the successful generation of monoclonal antibody (mAb)-producing hybridoma cell lines against target antigens.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kohler G, Milstein C (1975) Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256:495–497
Kohler G, Howe SC, Milstein C (1976) Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur J Immunol 6:292–295
Galfre G, Howe SC, Milstein C et al (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature 266:550–552
Shulman M, Wilde CD, Kohler G (1978) A better cell line for making hybridomas secreting specific antibodies. Nature 276:269–270
Galfre G, Milstein C (1981) Preparation of monolconal antibodies: strategies and procedures. Methods Enzymol 73B:3–46
Hurrell JGR (1982) Monoclonal hybridoma antibodies: techniques and applications. CRC Press, Boca Raton, FL
Schreier M, Kohler G, Hengartner H et al (1980) Hybridoma Techniques: EMBO, SKMB Course 1980, Basel. Cold Spring Harbor, New York
Golde WT, Gollobin P, Rodriguez LL (2005) A rapid, simple, and humane method for submandibular bleeding of mice using a lancet. Lab Anim 9:39–43
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Hnasko, R.M., Stanker, L.H. (2015). Hybridoma Technology. In: Hnasko, R. (eds) ELISA. Methods in Molecular Biology, vol 1318. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2742-5_2
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2742-5_2
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2741-8
Online ISBN: 978-1-4939-2742-5
eBook Packages: Springer Protocols