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Multiplex ELISA Using Oligonucleotide Tethered Antibodies

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ELISA

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1318))

Abstract

Multiplex assays represent a new paradigm for diagnostics. The simultaneous measure of multiple analytes from a single sample is advantageous in creating disease-associated panels that enable more accurate prognosis or diagnosis of the disease state. Furthermore, multiplexing may reduce reagent consumption, sample requirements and labor thereby lowering the cost per test. Here we describe a novel multiplex immunoassay technology based upon creating microarrays in microtiter plates that are formed upon the self-assembly of oligonucleotide–antibody conjugates.

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Correspondence to Robert S. Matson .

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© 2015 Springer Science+Business Media New York

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Matson, R.S. (2015). Multiplex ELISA Using Oligonucleotide Tethered Antibodies. In: Hnasko, R. (eds) ELISA. Methods in Molecular Biology, vol 1318. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2742-5_18

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  • DOI: https://doi.org/10.1007/978-1-4939-2742-5_18

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2741-8

  • Online ISBN: 978-1-4939-2742-5

  • eBook Packages: Springer Protocols

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