Abstract
Multiplexing is an important tool in assay development as it allows simultaneous detection of numerous analytes. Current platforms with the capability to multiplex are often complex and expensive. Here, we describe a low-cost planar array on a chip capable of simultaneously detecting up to 80 different analytes using the MagArray technology in as little as 10 min. This technology is easy to operate, has a small footprint, and is highly portable as it does not require any moving parts and/or microfluidics. This technology also allows the user to obtain a real-time read-out, which is very useful for analyzing complex sample matrices and for assessing cross-reactivity easily, or to monitor the dissociation of low affinity proteins during washes. The recommended sample volume for analysis is 100 μL after dilution, but as little as 20 μL can be measured if needed.
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References
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Acknowledgement
MagArray team also includes Sebastian J. Osterfeld, R. Adam Seger, Alice Juang, and Shan X. Wang (Stanford University). The authors would like to acknowledge the support of the NCI SBIR Bridge Award to MagArray (R44CA165296) and the Center for Cancer Nanotechnology Excellence to Stanford University (U54CA151459).
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© 2015 Springer Science+Business Media New York
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Clotilde, L.M., Yu, H., Carbonell, M.L. (2015). Multiplex Immunoassay: A Planar Array on a Chip Using the MagArray™ Technology. In: Hnasko, R. (eds) ELISA. Methods in Molecular Biology, vol 1318. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2742-5_12
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DOI: https://doi.org/10.1007/978-1-4939-2742-5_12
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2741-8
Online ISBN: 978-1-4939-2742-5
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