Abstract
Many proteins bind to nucleic acids. For the first characterization of novel proteins, a fast and simple technique for testing their nucleic acid binding capabilities is desirable. Here we describe the use of a North-western and South-western blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5).
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Acknowledgment
Special thanks for excellent technical support to Martina Franke (Inst. Immunology, Carl Gustav Carus TU-Dresden, Germany).
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Franke, C., Gräfe, D., Bartsch, H., Bachmann, M.P. (2015). Use of Nonradioactive Detection Method for North- and South-Western Blot. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_8
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DOI: https://doi.org/10.1007/978-1-4939-2718-0_8
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
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