Abstract
The use of nitrocellulose membranes for invisible ink-messaging or marking for orientation purposes has been carried out with diluted alkaline phosphatase, followed by development with nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate and chemiluminescence detection systems. Here, we show that nitrocellulose membrane can serve as a canvas for art using alkaline phosphatase and NBT/BCIP detection method.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Kurien BT (2009) Sending secret messages on nitrocellulose membrane and the use of a molecular pen for orientation in ECL membrane assays. Methods Mol Biol 536:573–581
Kurien BT, Scofield RH (eds) (2009) Protein blotting and detection: methods and protocols, vol 536, Methods in molecular biology. Springer, New York, USA
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Kurien, J.R., Kurien, B.A. (2015). Nitrocellulose Membrane: The New Canvas. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_37
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2718-0_37
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
eBook Packages: Springer Protocols