Abstract
Mucins are difficult to separate using conventional gel electrophoresis methods such as SDS-PAGE and agarose gel electrophoresis, owing to their large size and heterogeneity. On the other hand, cellulose acetate membrane electrophoresis can separate these molecules, but is not compatible with glycan analysis. Here, we describe a novel membrane electrophoresis technique, termed “supported molecular matrix electrophoresis” (SMME), in which a porous polyvinylidene difluoride (PVDF) membrane filter is used to achieve separation. This description includes the separation, visualization, and glycan analysis of mucins with the SMME technique.
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Acknowledgements
This work was performed as a part of the R&D project of the Industrial Science and Technology Frontier Program, which is supported by the New Energy and Industrial Technology Development Organization (NEDO). This work was also supported by a Grant-in-Aid for Scientific Research (KAKENHI 23310154) from the Japan Society for the Promotion of Science (JSPS).
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Matsuno, Yk., Kameyama, A. (2015). Supported Molecular Matrix Electrophoresis. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_31
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DOI: https://doi.org/10.1007/978-1-4939-2718-0_31
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2717-3
Online ISBN: 978-1-4939-2718-0
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