Abstract
We describe here a simple method of staining nitrocellulose/PVDF blots by Alta. This red-colored stain which is used as a cosmetic contains Crocein scarlet and Rhodamine B as the principal components. It is very cheap, is available as a ready-to-use liquid, and is as sensitive as the most commonly used stain Ponceau Red S. We further demonstrate that Crocein scarlet (one of the principal components) alone can be used for staining the blots with equal efficiency as well. The stained protein profile can be detected on a white light plate and documented in the usual manner. Detailed analysis indicates that this stain does not interfere with subsequent immunoreactions. Moreover, Alta is almost 100 times cheaper to the routinely used stain Ponceau Red S, and thus is an ideal alternative to the Ponceau Red S for staining blots during western blot analysis.
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Acknowledgements
This work is supported by a research grant from the Department of Science and Technology (DST), Govt. of India, under the DST PURSE Program, and the Department Research and Development grant of the Savitribai Phule Pune University.
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Pal, J.K., Rao, S.J., Godbole, D.J. (2015). Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta. In: Kurien, B., Scofield, R. (eds) Detection of Blotted Proteins. Methods in Molecular Biology, vol 1314. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2718-0_29
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DOI: https://doi.org/10.1007/978-1-4939-2718-0_29
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