Detection of Fusion Events in Mammalian Skeletal Muscle

Abstract

Cell fusion events are essential for the maintenance of skeletal muscle tissue and during its repair processes after damage. However, these mechanisms have not come much into focus in the recent years. Different methods can be used to assess ongoing cell fusion events in adult skeletal muscle tissue. Among these methods, confocal microscopy, western blotting, and quantitative polymerase chain reactions are ideal, since they provide concerted information about cell fusion events going on in skeletal muscle tissue at both qualitative and quantitative levels. Confocal microscopy allows for the visualization of exact localizations of cell fusion events in adult skeletal muscle. Western blotting allows for a semiquantitative evaluation of protein levels involved and associated with cell fusions events. Finally, quantitative polymerase chain reaction is a valuable tool to precisely assess mRNA levels of genes involved and associated with cell fusions events. In addition to the investigation if cell fusion markers in skeletal muscle tissue, in vitro cell culture systems (e.g., C2C12 cells) can be used to study cell fusions events in a highly standardized system in order to obtain detailed information about genes and proteins involved in these processes. Here, confocal microscopy, western blotting, and quantitative polymerase chain reaction are described as methods to investigate cell fusion events and how a C2C12 cell culture system can be run to support the studies of adult muscle tissue.