Abstract
Auxiliary use of mesenchymal stem/stromal cells (MSCs) to islet transplantation is shown to enhance efficacy. We hypothesized cell fusion of islet cells and MSCs may provide a new cell source with robustness of MSCs and islet cell function. We succeeded electrofusion between dispersed islet cells and MSCs in rats and fused cells sustained beta-cell function in vitro and in vivo, suggesting their possibility of therapeutic application. Here, we describe our method of cell fusion that enabled us to fuse islet cells to MSCs.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Borg DJ, Weigelt M, Wilhelm C, Gerlach M, Bickle M, Speier S, Bonifacio E, Hommel A (2014) Mesenchymal stromal cells improve transplanted islet survival and islet function in a syngeneic mouse model. Diabetologia 57:522–531
Rackham CL, Dhadda PK, Chagastelles PC, Simpson SJ, Dattani AA, Bowe JE, Jones PM, King AJ (2013) Pre-culturing islets with mesenchymal stromal cells using a direct contact configuration is beneficial for transplantation outcome in diabetic mice. Cytotherapy 15:449–459
Yeung TY, Seeberger KL, Kin T, Adesida A, Jomha N, Shapiro AM, Korbutt GS (2012) Human mesenchymal stem cells protect human islets from pro-inflammatory cytokines. PLoS One 7:e38189
Stagg J, Galipeau J (2013) Mechanisms of immune modulation by mesenchymal stromal cells and clinical translation. Curr Mol Med 13:856–867
Marappagounder D, Somasundaram I, Dorairaj S, Sankaran RJ (2013) Differentiation of mesenchymal stem cells derived from human bone marrow and subcutaneous adipose tissue into pancreatic islet-like clusters in vitro. Cell Mol Biol Lett 18:75–88
Harris H (1967) The Reactivation of the red cell nucleus. J Cell Sci 2:23–32
Tada M, Takahama Y, Abe K, Nakatsuji N, Tada T (2001) Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr Biol 11:1553–1558
Soza-Ried J, Fisher AG (2012) Reprogramming somatic cells towards pluripotency by cellular fusion. Curr Opin Genet Dev 22:459–465
Gurdon JB, Melton DA (2008) Nuclear reprogramming in cells. Science 322:1811–1815
Yanai G, Hayashi T, Zhi Q, Yang KC, Shirouzu Y, Shimabukuro T, Hiura A, Inoue K, Sumi S (2013) Electrofusion of mesenchymal stem cells and islet cells for diabetes therapy: a rat model. PLoS One 8:e64499
Hayashi T, Tanaka H, Tanaka J, Wang R, Averbook BJ, Cohen PA, Shu S (2002) Immunogenicity and therapeutic efficacy of dendritic-tumor hybrid cells generated by electrofusion. Clin Immunol 104:14–20
Neil GA, Zimmermann U (1993) Electrofusion. Methods Enzymol 220:174–196
Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Dj P, Horwitz E (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317
Harting MT, Jimenez F, Pati S, Baumgartner J, Cox CS (2008) Immunophenotype characterization of rat mesenchymal stromal cells. Cytotherapy 10:243–253
Acknowledgement
This work was partly supported by JSPS KAKENHI Grant Number 25461943.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Sumi, S., Yanai, G. (2015). Fusion of Mesenchymal Stem Cells and Islet Cells for Cell Therapy. In: Pfannkuche, K. (eds) Cell Fusion. Methods in Molecular Biology, vol 1313. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2703-6_7
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2703-6_7
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2702-9
Online ISBN: 978-1-4939-2703-6
eBook Packages: Springer Protocols