Abstract
Plant transcriptional responses to gravity stimulation by reorientation are among the fastest measured in any tissue or species. Upon reorientation, changes in abundance of specific mRNAs can be measured within seconds or minutes, for plastid or nuclear encoded genes, respectively. Identifying fast gravity-induced transcripts has been made possible by the development of high-throughput technology for qualitative and quantitative RNA analysis. RNA profiling has undergone further rapid development due to its enormous potential in basic sciences and medical applications. We describe here the current and most widely used methods to profile the changes in an entire transcriptome by high-throughput sequencing of RNA fractions (RNAseq) and single gene transcript analysis using real-time quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR).
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Funding for transcript profiling in our labs was provided by NASA, NSF, DOE, and USDA.
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Dalal, J. et al. (2015). Methods for RNA Profiling of Gravi-Responding Plant Tissues. In: Blancaflor, E. (eds) Plant Gravitropism. Methods in Molecular Biology, vol 1309. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2697-8_9
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DOI: https://doi.org/10.1007/978-1-4939-2697-8_9
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2696-1
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