Abstract
Gravitropic signaling is a complex process that requires the coordinated action of multiple cell types and tissues. Ca2+ and pH signaling are key components of gravitropic signaling cascades and can serve as useful markers to dissect the molecular machinery mediating plant gravitropism. To monitor dynamic ion signaling, imaging approaches combining fluorescent ion sensors and confocal fluorescence microscopy are employed, which allow the visualization of pH and Ca2+ changes at the level of entire tissues, while also providing high spatiotemporal resolution. Here, I describe procedures to prepare Arabidopsis seedlings for live cell imaging and to convert a microscope for vertical stage fluorescence microscopy. With this imaging system, ion signaling can be monitored during all phases of the root gravitropic response.
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Acknowledgements
The work on root ion signaling in the author’s laboratory is supported by grants from the National Science Foundation (MCB-1121994) and NASA (NNX13AM47G).
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Monshausen, G.B. (2015). Imaging of Dynamic Ion Signaling During Root Gravitropism. In: Blancaflor, E. (eds) Plant Gravitropism. Methods in Molecular Biology, vol 1309. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2697-8_5
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DOI: https://doi.org/10.1007/978-1-4939-2697-8_5
Publisher Name: Humana Press, New York, NY
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