Abstract
P-selectin is a multi-domain glycoprotein expressed on activated endothelial cells and activated platelets. We previously expressed a recombinant form of P-selectin containing only its N-terminal lectin and EGF domains in CHO-K1 cells and showed that these two domains are sufficient to mediate ligand binding. We have now expressed the same construct in CHO-Lec1 cells that make truncated glycans. The uniform glycosylation in these cells should make it easier to crystallize this protein.
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Acknowledgement
The author thanks Dr. Rodger P. McEver for the soluble, recombinant P-selectin and Dr. Charles Esmon for the HPC4 antibody.
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Mehta-D’souza, P. (2015). Immunodetection of P-Selectin Using an Antibody to Its C-Terminal Tag. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_31
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DOI: https://doi.org/10.1007/978-1-4939-2694-7_31
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2693-0
Online ISBN: 978-1-4939-2694-7
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