Abstract
The history of the development of DNA blotting is described in this chapter. DNA blotting, involving the transfer of electrophoretically separated DNA fragments to a membrane support through capillary action, is also known as Southern blotting. This procedure enables the detection of a specific DNA sequence by hybridization with probes. The term Southern blotting led to a “geographic” naming tradition, with RNA blotting bearing the name Northern blotting and protein transfer to membranes becoming known as Western blotting.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ford PJ, Southern EM (1973) Different sequences for 5S RNA in kidney cells and ovaries of Xenopus laevis. Nat New Biol 241:7–12
Kelly TJ, Smith HO (1970) A restriction enzyme from Hemophilus influenzae. II. J Mol Biol 51:393–409
Shinnick TM, Lund E, Smithies O, Blattner FR (1975) Hybridization of labeled RNA to DNA in agarose gels. Nucleic Acids Res 10:1911–1929
Chen CW, Thomas CA Jr (1980) A rapid two-dimensional fractionation of DNA restriction fragments. Anal Biochem 101:339–341
Gillespie D, Spiegelman S (1965) A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol 12:829–842
Sakonju S, Bogenhagen DF, Brown DD (1980) A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the region. Cell 19:13–25
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Southern, E. (2015). The Early Days of Blotting. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_1
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2694-7_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2693-0
Online ISBN: 978-1-4939-2694-7
eBook Packages: Springer Protocols