Abstract
Over the past few years algal extracts have become increasingly interesting to the scientific community due to their promising biological properties. Phlorotannin extracts are particularly attractive partly due to their reported antifungal activity against several yeast and dermatophyte strains.
The micromethod used for the evaluation of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) represents an effective and solvent-saving procedure to evaluate the antifungal activity of algae extracts. Here we describe the micromethod for determining the MIC and the MLC of algal extracts by using the example of a purified phlorotannin extract of brown algae.
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References
Mohamed S, Hashim SN, Rahman A (2012) Seaweeds: a sustainable functional food for complementary and alternative therapy. Trends Food Sci Technol 23:83–96
Blunt JW, Copp BR, Munro MHG et al (2012) Marine natural products. Nat Prod Rep 29:144–222
Shibata T, Kawaguchi S, Hama Y et al (2004) Local and chemical distribution of phlorotannins in brown algae. J Appl Phycol 16:291–296
Koivikko R, Loponen J, Honkanen T et al (2005) Contents of soluble, cell-wall-bound and exuded phlorotannins in the brown algae Fucus vesiculosus, with implications on their ecological functions. J Chem Ecol 31:195–212
Kim AR, Shi TS, Lee MS et al (2009) Isolation and identification of phlorotannins from Ecklonia stolonifera with antioxidant and anti-inflammatory properties. J Agric Food Chem 57:3483–3489
Genovese G, Leithner S, Minicante S et al (2013) The Mediterranean red alga Asparagopsis taxiformis has antifungal activity against Aspergillus species. Mycoses 56:516–519
Mayer AMS, Rodríguez AD, Taglialatela-Scafati O et al (2013) Marine pharmacology in 2009-2011: marine compounds with antibacterial, antidiabetic, antifungal, anti-inflammatory, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous systems and other miscellaneous mechanisms of action. Mar Drugs 11:2510–2573
Lopes G, Sousa C, Silva LR et al (2012) Can phlorotannins purified extracts constitute a novel pharmacological alternative for microbial infections with associated inflammatory conditions? PLoS One 7:e31145
Ferreres F, Lopes G, Gil-Izquierdo A et al (2012) Phlorotannin extracts from Fucales characterized by HPLC-DAD-ESI-MSn: approaches to hyaluronidase inhibitory capacity and antioxidant properties. Mar Drugs 10:2766–2781
Li Y, Qian ZJ, Ryu B et al (2009) Chemical components and its antioxidant properties in vitro: an edible marine brown alga, Ecklonia cava. Bioorg Med Chem 17:1963–1973
Parys S, Kehraus S, Krick S et al (2010) In vitro chemopreventive potential of fucophlorethols from the brown alga Fucus vesiculosus L. by antioxidant activity of selected cytochrome P450 enzymes. Phytochemistry 71:221–229
Devi KP, Suganthy N, Kesika P et al (2008) Bioprotective properties of seaweeds: in vitro evaluation of antioxidant activity and antimicrobial activity against food borne bacteria in relation to polyphenolic content. BMC Complement Altern Med 8:38
Cox S, Abu-Ghannam N, Gupta S (2010) An assessment of the antioxidant and antimicrobial activity of six species of edible Irish seaweeds. Int Food Res J 17:205–220
Eom SH, Kim YW, Kim S-K (2012) Antimicrobial effect of phlorotannins from marine brown algae. Food Chem Toxicol 50:3251–3255
Lopes G, Pinto E, Andrade PB et al (2013) Antifungal activity of phlorotannins against dermatophyte and yeast: approach to the mechanism of action and influence on Candida albicans virulence factor. PLoS One 8:e72203
Lee MH, Lee KB, Oh SM et al (2010) Antifungal activities of dieckol isolated from the marine brown alga Ecklonia cava against Trichophyton rubrum. J Korean Soc Appl Biol Chem 53:504–507
Richardson MD, Warnock DW (2012) Fungal infection: diagnosis and management, 4th edn. Wiley-Blackwell, Oxford, UK
Ghannoum MA, Rice LB (1999) Antifungal agents: mode of action, mechanisms of resistance and correlation of these mechanisms with bacterial resistance. Clin Microbiol Rev 12:501–517
Clinical and Laboratory Standard Institute (CLSI) (2008) Reference method for broth dilution antifungal susceptibility testing of filamentous fungi: approved standard, 2nd ed. M38-A2, Wayne, PA
Clinical and Laboratory Standard Institute (CLSI) (2008) Reference method for broth dilution antifungal susceptibility testing of yeasts: approved standard, 3rd ed. M27-A3, Wayne, PA
Coenye T, Vos MD, Vanderbosch D et al (2008) Factors influencing the trailing endpoint observed in Candida albicans susceptibility testing using the CLSI procedure. Clin Microbiol Infect 14:495–497
Fairhead VA, Amsler CD, McClintock JB et al (2005) Variation in phlorotannin content within two species of brown macroalgae (Desmarestia anceps and D. menziesii) from the Western Antarctic Peninsula. Polar Biol 28:680–686
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Lopes, G., Andrade, P.B., Valentão, P. (2015). Screening of a Marine Algal Extract for Antifungal Activities. In: Stengel, D., Connan, S. (eds) Natural Products From Marine Algae. Methods in Molecular Biology, vol 1308. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2684-8_26
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DOI: https://doi.org/10.1007/978-1-4939-2684-8_26
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2683-1
Online ISBN: 978-1-4939-2684-8
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