Abstract
Sulfated and pyruvylated galactans are the major soluble polysaccharides produced by seaweeds of the Bryopsidales. Their backbones have a complex and variable pattern of substitution which, until now, has only been elucidated for a few species. Methods for determination of sulfate and pyruvic acid content, and chemical strategies to determine their position in the galactan chain are outlined here. These methods can also be applied to other sulfated and/or pyruvylated polysaccharides.
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Acknowledgements
This work was supported by grants from National Research Council of Argentina, CONICET (PIP 559-2009), and the National Agency for Promotion of Science and Technology, ANPCYT (PICT 2008-0500). PVF and MC are Research Members of the National Research Council of Argentina (CONICET).
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Arata, P.X., Fernández, P.V., Ciancia, M. (2015). Determination of Substitution Patterns of Galactans from Green Seaweeds of the Bryopsidales. In: Stengel, D., Connan, S. (eds) Natural Products From Marine Algae. Methods in Molecular Biology, vol 1308. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2684-8_20
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DOI: https://doi.org/10.1007/978-1-4939-2684-8_20
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