Abstract
Protein kinases regulate signaling pathways by phosphorylating their targets. They play critical roles in plant signaling networks. Although many important protein kinases have been identified in plants, their substrates are largely unknown. We have developed and produced plant protein microarrays with more than 15,000 purified plant proteins. Here, we describe a detailed protocol to use these microarrays to identify plant protein kinase substrates via in vitro phosphorylation assays on these arrays.
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Acknowledgement
The protein microarray work in SPD-K lab was supported by National Science Foundation grants DBI-0723722 and DBI-1042344 and currently by UC Davis funds.
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Ma, S., Dinesh-Kumar, S.P. (2015). Plant Protein Kinase Substrates Identification Using Protein Microarrays. In: Schulze, W. (eds) Plant Phosphoproteomics. Methods in Molecular Biology, vol 1306. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2648-0_12
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DOI: https://doi.org/10.1007/978-1-4939-2648-0_12
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2647-3
Online ISBN: 978-1-4939-2648-0
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