Abstract
Superoxide (O2 −) and nitric oxide (NO) are produced within plant mitochondria and may have signaling functions within the cell. Here we describe semiquantitative fluorescence imaging-based approaches to estimate the mitochondrial amount of these reactive and short-lived species within intact leaf tissue. We also outline a biochemical method using oxyhemoglobin to measure NO within a whole leaf tissue extract. This quantitative method, while not specifically evaluating mitochondrial localized NO, does nonetheless provide another independent measure of NO that can be useful.
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Cvetkovska, M., Vanlerberghe, G.C. (2015). In Planta Analysis of Leaf Mitochondrial Superoxide and Nitric Oxide. In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_18
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DOI: https://doi.org/10.1007/978-1-4939-2639-8_18
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2638-1
Online ISBN: 978-1-4939-2639-8
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