Abstract
In plants, apoptosis-like programmed cell death (AL-PCD) is readily distinguished from other forms of programmed cell death (PCD) through a distinct morphology. Detection of cytochrome c release from mitochondria and changes in mitochondrial morphology are the earliest markers for detection of this form of PCD in plants. In this chapter we provide detailed technical methods for the visualization of both of these mitochondrial markers of AL-PCD in Arabidopsis.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lam E (2004) Controlled cell death, plant survival and development. Nat Rev Mol Cell Biol 5:305–315
Reape TJ, Molony EM, McCabe PF (2008) Programmed cell death in plants: distinguishing between different modes. J Exp Bot 59:435–444
Reape TJ, McCabe PF (2008) Apoptotic-like programmed cell death in plants. New Phytol 180:13–26
McCabe PF, Leaver CJ (2000) Programmed cell death in cell cultures. Plant Mol Biol 44:359–368
Burbridge E, Diamond M, Dix PJ, McCabe PF (2006) Use of cell morphology to evaluate the effect of a peroxidase gene on cell death induction thresholds in tobacco. Plant Sci 171:139–146
Diamond M, Reape TJ, Rocha O, Doyle SM, Kacprzyk J, Doohan F, McCabe PF (2013) The Fusarium mycotoxin deoxynivalenol can inhibit plant apoptosis-like programmed cell death. PLoS One 8(7):e69542
Hogg BV, Kacprzyk J, Molony EM, O’Reilly C, Gallagher TF, Gallois P, McCabe PF (2011) An in vivo root hair assay for determining rates of apoptotic-like programmed cell death in plants. Plant Methods 7:45
Kacprzyk J, Devine A, McCabe PF (2014) The root hair assay facilitates the use of genetic and pharmacological tools in order to dissect multiple signalling pathways that lead to programmed cell death. PLoS One 9(4):e94898
Scott I, Logan DC (2008) Mitochondrial transition morphology is an early indicator of subsequent cell death in Arabidopsis. New Phytol 177:90–101
Balk J, Leaver CJ, McCabe PF (1999) Translocation of cytochrome c from the mitochondria to the cytosol occurs during heat-induced programmed cell death in cucumber plants. FEBS Lett 463:151–154
Balk J, Chew SK, Leaver CJ, McCabe PF (2003) The intermembrane space of plant mitochondria contains a DNase activity that may be involved in programmed cell death. Plant J 34:573–583
May MJ, Leaver CJ (1993) Oxidative stimulation of glutathione synthesis in Arabidopsis thaliana suspension cultures. Plant Physiol 103:621–627
Pendergrass W, Wolf N, Poot M (2004) Efficacy of MitoTracker Green™ and CMX rosamine to measure changes in mitochondrial membrane potentials in living cells and tissues. Cytometry A 61A:162–169
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Sweetlove LJ, Taylor NL, Leaver CJ (2007) Isolation of intact, functional mitochondria from the model plant Arabidopsis thaliana. In: Leister D, Hermann JM (eds) Methods Mol Biol, vol 372. Humana, Totowa, NJ, pp 125–136
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Reape, T.J., Kacprzyk, J., Brogan, N., Sweetlove, L., McCabe, P.F. (2015). Mitochondrial Markers of Programmed Cell Death in Arabidopsis thaliana . In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2639-8_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2638-1
Online ISBN: 978-1-4939-2639-8
eBook Packages: Springer Protocols