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Analysis of Type II NAD(P)H Dehydrogenases

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Plant Mitochondria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1305))

Abstract

Plant mitochondria contain at least four type II NAD(P)H dehydrogenases that link NAD(P)H oxidation to the inner membrane electron transport chain and bypass proton pumping at Complex I, hence ATP synthesis. These activities have been found in mitochondria isolated from all plant species analyzed to date. In this chapter, methods are presented to analyze the expression of genes encoding these dehydrogenases and to detect protein levels in mitochondria isolated from Arabidopsis (Arabidopsis thaliana). In addition, methods and assay conditions are presented to detect the activity of each of these four type II NAD(P)H dehydrogenases in isolated plant mitochondria.

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Author information

Authors and Affiliations

  1. School of Biological Sciences, Flinders University, Sturt Road, Bedford Park 5042, GPO Box 2100, Adelaide, 5001, SA, Australia

    Kathleen L. Soole & Chevaun A. Smith

Authors
  1. Kathleen L. Soole
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  2. Chevaun A. Smith
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Corresponding author

Correspondence to Kathleen L. Soole .

Editor information

Editors and Affiliations

  1. La Trobe University, Bundoora, Victoria, Australia

    James Whelan

  2. The University of Western Australia, Crawley, West Australia, Australia

    Monika W. Murcha

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Soole, K.L., Smith, C.A. (2015). Analysis of Type II NAD(P)H Dehydrogenases. In: Whelan, J., Murcha, M. (eds) Plant Mitochondria. Methods in Molecular Biology, vol 1305. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2639-8_11

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  • DOI: https://doi.org/10.1007/978-1-4939-2639-8_11

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2638-1

  • Online ISBN: 978-1-4939-2639-8

  • eBook Packages: Springer Protocols

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