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Detection of Nepovirus Vector and Nonvector Xiphinema Species in Grapevine

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Plant Pathology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1302))

Abstract

Fanleaf degeneration is considered the most damaging viral disease of grapevine. The two major nepoviruses involved are Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV) which are respectively and specifically transmitted by the dagger nematodes Xiphinema index and X. diversicaudatum. The methods described below are aimed at detecting four prevalent grapevine Xiphinema species: the vector species previously mentioned and two nonvector species X. vuittenezi and X. italiae.

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Acknowledgments

This study was made possible by the support of SASA (Science and Advice for Scottish Agriculture) facilities (Edinburgh, Scotland, UK) through the “Diagnostics, Wildlife & Molecular Biology” section.

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Correspondence to C. Van Ghelder .

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Van Ghelder, C., Reid, A., Kenyon, D., Esmenjaud, D. (2015). Detection of Nepovirus Vector and Nonvector Xiphinema Species in Grapevine. In: Lacomme, C. (eds) Plant Pathology. Methods in Molecular Biology, vol 1302. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2620-6_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2620-6_12

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2619-0

  • Online ISBN: 978-1-4939-2620-6

  • eBook Packages: Springer Protocols

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