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Label-Free Cell Phenotypic Profiling and Screening: Techniques, Experimental Design, and Data Assessment

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Label-Free Biosensor Methods in Drug Discovery

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Label-free biosensors enable novel cell phenotypic assays for drug discovery by providing a holistic view of drug action in native cells. The label-free cellular profiles of drug molecules permit the comprehension of their target(s), potency, efficacy, and safety. This chapter first discusses three essential components of label-free cell phenotypic assays, namely biosensors, cell phenotypes, and assays. Key considerations about experimental design, data quality assessment, and data analysis are then discussed.

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Authors and Affiliations

  1. Science and Technology Division, Biochemical Technologies, Corning Incorporated, Corning, NY, 14831, USA

    Ye Fang

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Correspondence to Ye Fang .

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  1. Biochemical Technologies, Science and Technology Division, Corning Incorporated, Corning, New York, USA

    Ye Fang

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Fang, Y. (2015). Label-Free Cell Phenotypic Profiling and Screening: Techniques, Experimental Design, and Data Assessment. In: Fang, Y. (eds) Label-Free Biosensor Methods in Drug Discovery. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2617-6_2

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  • DOI: https://doi.org/10.1007/978-1-4939-2617-6_2

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2616-9

  • Online ISBN: 978-1-4939-2617-6

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