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Pulse Field Gel Electrophoresis pp 139–156Cite as

Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1301))

Abstract

Pulsed field gel electrophoresis (PFGE) is generally accepted as one of the most discriminatory methods available for genotyping Campylobacter jejuni. PFGE has been extensively used in epidemiological studies, including outbreak investigation, persistence of genotypes in a human population, environmental diversity of sporadic infection isolates, dissemination of antibiotic-resistant strains, and comparison of genotypes within and between hosts. The main purpose of this chapter is to present a working PFGE protocol for those interested in incorporating this technique in their laboratories.

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Author information

Authors and Affiliations

  1. Department of Neurology, University of Alabama at Birmingham, 1720 7th Avenue South, Birmingham, AL, 35233, USA

    Ping Zhou

  2. IEH Laboratories and Consulting Group, Lake Forest Park, WA, USA

    Omar A. Oyarzabal

  3. University of Vermont Extension, 617 Comstock Road, Suite 5, Berlin, VT, 05602, USA

    Omar A. Oyarzabal

Authors
  1. Ping Zhou
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  2. Omar A. Oyarzabal
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Corresponding author

Correspondence to Omar A. Oyarzabal .

Editor information

Editors and Affiliations

  1. Teagasc Food Research Center, Moorepark, Fermoy, Co. Cork, Cork, Ireland

    Kieran Jordan

  2. Department of Microbiology & Alimentary Pharmabiotic Centre, University College Cork, Co. Cork, Cork, Ireland

    Marion Dalmasso

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Zhou, P., Oyarzabal, O.A. (2015). Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni . In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_13

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  • DOI: https://doi.org/10.1007/978-1-4939-2599-5_13

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2598-8

  • Online ISBN: 978-1-4939-2599-5

  • eBook Packages: Springer Protocols

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