Abstract
In this chapter, we describe in detail how to prepare a sample containing the complete entity of secretion products from murine primary adipocytes, which are suitable for comprehensive and sensitive secretome analysis. The underlying protocol should be seen as a starting point guiding through critical steps of the complex workflow in order to approximate to the real secretome in the context of different sample types used for the diverse research questions the protocol has to be carefully adjusted.
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Göddeke, S., Kotzka, J., Lehr, S. (2015). Investigating the Adipose Tissue Secretome: A Protocol to Generate High-Quality Samples Appropriate for Comprehensive Proteomic Profiling. In: Posch, A. (eds) Proteomic Profiling. Methods in Molecular Biology, vol 1295. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2550-6_4
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DOI: https://doi.org/10.1007/978-1-4939-2550-6_4
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2549-0
Online ISBN: 978-1-4939-2550-6
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