Abstract
This chapter covers the various methods of Mechanical Cell Disruption and Tissue Homogenization that are currently commercially available for processing minute samples (<1 mL) to larger production quantities. These mechanical methods of lysing do not introduce chemicals or enzymes to the system. However, the energies needed when using these “harsh” methods can be high and destroy the very proteins being sought.
The destruction of cell membranes and walls by these “harsh” methods is effected by subjecting the cells (1) to shearing by liquid flow, (2) to exploding by pressure differences between inside and outside of cell, (3) to collision forces by impact of beads or paddles, or (4) a combination of these forces. Practical suggestions to optimize each method, where to acquire such equipment, and links to reference sources are included.
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Acknowledgements
Superb assistance was rendered by the following people who are most conversant in their noted equipment areas. Bead Milling by Tim Hopkins (Biospec Products) and Harald Frommherz (W. A. Bachofen AG); Rotor-Stator by Roger Munsinger (Kinematica USA/AG); High pressure flowing liquid by Toni Parker (SPX) and Steven Mesite (Microfluidics/IDEX); High pressure batch liquid by Robert Borgon (University of Central Florida); High pressure batch gas by Kay Frere and Lisa Randolph (Parr Instruments); Low pressure flowing gas by James Jacso (Glas-Col); and Ultrasonic processors by Andrea Coppola and Marc Lusting (QSonica); Electro Water Separation by Devin Angus and Jean-Louis Kindle (Origin Oil).
My book editor Dr. Anthon Posch has been exceptionally supportive in both technical as well as motivational matters. My wife Robin and sons Evan and Adam are the sparkle of my life.
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Goldberg, S. (2015). Mechanical/Physical Methods of Cell Distribution and Tissue Homogenization. In: Posch, A. (eds) Proteomic Profiling. Methods in Molecular Biology, vol 1295. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2550-6_1
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DOI: https://doi.org/10.1007/978-1-4939-2550-6_1
Publisher Name: Humana Press, New York, NY
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