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Direct Cloning of Double-Stranded RNAs

  • Protocol
Small Non-Coding RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1296))

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Abstract

Most annotated genomes show a large number of sense–antisense transcripts that can generate double-stranded RNAs. We describe a method to clone these dsRNAs from total RNA preparations.

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Acknowledgment

This work was supported by GM083187 and P20GM1034865-10 from the NIH and an endowment of the University of Kentucky.

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Authors and Affiliations

  1. University of Kentucky, Lexington, KY, 40536, USA

    Manli Shen, Marina Falaleeva, Natalia Korotkova & Stefan Stamm

Authors
  1. Manli Shen
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  2. Marina Falaleeva
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  3. Natalia Korotkova
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  4. Stefan Stamm
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Corresponding author

Correspondence to Stefan Stamm .

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Editors and Affiliations

  1. Université de Lorraine, Vandoeuvre-lès-Nancy, France

    Mathieu Rederstorff

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Shen, M., Falaleeva, M., Korotkova, N., Stamm, S. (2015). Direct Cloning of Double-Stranded RNAs. In: Rederstorff, M. (eds) Small Non-Coding RNAs. Methods in Molecular Biology, vol 1296. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2547-6_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2547-6_6

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2546-9

  • Online ISBN: 978-1-4939-2547-6

  • eBook Packages: Springer Protocols

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