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cAMP Signaling pp 71–84Cite as

Channel-Based Reporters for cAMP Detection

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1294))

Abstract

In the last 15 years, tremendous progress has been made in the development of single-cell cAMP sensors. Sensors are based upon cAMP-binding proteins that have been modified to transduce cAMP concentrations into electrical or fluorescent readouts that can be readily detected using patch clamp amplifiers, photomultiplier tubes, or cameras. Here we describe two complementary approaches for the detection and measurement of cAMP signals near the plasma membrane of cells. These probes take advantage of the ability of cyclic nucleotide-gated (CNG) channels to transduce small changes in cAMP concentrations into ionic flux through channel pores that can be readily detected by measuring Ca2+ and/or Mn2+ influx or by measuring ionic currents.

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Acknowledgments

This work was supported by NIH P01HL066299, the Center for Lung Biology, and the Colleges of Medicine and Engineering, University of South Alabama.

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Authors and Affiliations

  1. Department of Pharmacology, University of South Alabama, MSB 3370, 5851 USA Drive North, Mobile, AL, 36688, USA

    Thomas C. Rich & Silas J. Leavesley

  2. Center for Lung Biology, University of South Alabama, Mobile, AL, USA

    Thomas C. Rich & Silas J. Leavesley

  3. Department of Drug Discovery and Biomedical Sciences, South Carolina College of Pharmacy, Mobile, AL, USA

    Wenkuan Xin

  4. Department of Chemical and Biomolecular Engineering, University of South Alabama, Mobile, AL, USA

    Silas J. Leavesley

  5. Department of Physiology, University of South Alabama, Mobile, AL, USA

    Mark S. Taylor

Authors
  1. Thomas C. Rich
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  2. Wenkuan Xin
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  3. Silas J. Leavesley
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  4. Mark S. Taylor
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Corresponding author

Correspondence to Thomas C. Rich .

Editor information

Editors and Affiliations

  1. Department of Physiology, Anatomy, and Genetics, University of Oxford, Oxford, United Kingdom

    Manuela Zaccolo

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Rich, T.C., Xin, W., Leavesley, S.J., Taylor, M.S. (2015). Channel-Based Reporters for cAMP Detection. In: Zaccolo, M. (eds) cAMP Signaling. Methods in Molecular Biology, vol 1294. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2537-7_6

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  • DOI: https://doi.org/10.1007/978-1-4939-2537-7_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2536-0

  • Online ISBN: 978-1-4939-2537-7

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