Abstract
Lentiviral vectors are the workhorses of modern cell biology. They can infect a wide variety of cells including nondividing cells and stem cells. They integrate into the genome of infected cells leading to stable expression. It is easy to transduce 100 % of the cells in a culture and possible to infect cells simultaneously with multiple vectors, greatly facilitating studies on malignant transformation. We present simple protocols to produce and titrate lentiviral vectors, infect mammary epithelial cells, and check for contamination with replication-competent viruses.
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Notes
- 1.
Additional useful information can be found on http://tronolab.epfl.ch/lentivectors and http://www.addgene.org.
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Additional useful information can be found on http://tronolab.epfl.ch/lentivectors and http://www.addgene.org.
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Acknowledgements
We thank former members of the lab, in particular Stephan Duss, for help in developing these protocols and the French Cancer Ligue (“Equipe Labellisee Ligue Contre le Cancer 2011”) for financial support.
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Iggo, R., Richard, E. (2015). Lentiviral Transduction of Mammary Epithelial Cells. In: Vivanco, M. (eds) Mammary Stem Cells. Methods in Molecular Biology, vol 1293. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2519-3_8
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DOI: https://doi.org/10.1007/978-1-4939-2519-3_8
Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-2519-3
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