Abstract
Single-cell genome and transcriptome characterizations will probe to be decisive within the stem cells research, especially to describe appropriately the genetic impact of the diverse stem cells populations that are present in each organism. In the present chapter, we describe in detail how to prepare sequencing libraries out of single cells, for whole genome DNA and mRNA sequencing.
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Acknowledgements
We would like to thank Juan José Fuentes Nuño for his assistance on single-cell isolation procedure description. A.G.L. and some research expenses are partially supported by CIBERehd. A.M.A., N.M., A.G.L., L.B., and research expenses are supported by the Basque Country Government (Etortek Research Programs 2010/2014) and Bizkaia County’s Innovation Technology Department.
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Aransay, A.M., Barcena, L., Gonzalez-Lahera, A., Macias-Camara, N. (2015). Single-Cell Genome and Transcriptome Processing Prior to High-Throughput Sequencing. In: Vivanco, M. (eds) Mammary Stem Cells. Methods in Molecular Biology, vol 1293. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2519-3_5
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DOI: https://doi.org/10.1007/978-1-4939-2519-3_5
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2518-6
Online ISBN: 978-1-4939-2519-3
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